fetal lung fibroblast cell line hfl1  (ATCC)

Journal: Cells

Article Title: Systematic Comparison of the TGF-β Isoforms in Normal Dermal and Lung Fibroblasts Identifies TGF-β2 and TGF-β3 as Priority Targets in Tissue Fibrosis

doi: 10.3390/cells15080671

Figure Lengend Snippet: Study methodology. Fibroblast cell lines derived from neonatal skin and fetal lung tissue were stimulated with TGF-β isoforms 1, 2, and 3 at 10 ng/mL. Cells were phenotyped by Western blotting to assess extracellular matrix proteins, signalling molecules, and myofibroblast differentiation, ELISAs to measure inflammatory and remodelling cytokines, collagen-I matrix assays to assess fibroblast contraction, and a proliferation assay to assess the effect of the TGF-β isoforms on cell accumulation (Created in BioRender 201).

Article Snippet: As shown in , the study design used neonatal dermal fibroblasts (BJ, Cat: CRL-2522) and fetal lung fibroblasts (HFL1, Cat: CCL-153) obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA), which are commonly used human, non-transformed dermal and lung fibroblast cell lines.

Techniques: Derivative Assay, Western Blot, Proliferation Assay

Journal: Cells

Article Title: Systematic Comparison of the TGF-β Isoforms in Normal Dermal and Lung Fibroblasts Identifies TGF-β2 and TGF-β3 as Priority Targets in Tissue Fibrosis

doi: 10.3390/cells15080671

Figure Lengend Snippet: TGF-β2 and TGF-β3 are strong inducers of profibrotic IL-11 and IL-6 release by dermal and lung fibroblasts. Dermal and lung fibroblasts ( n = 11 biological replicates for dermal fibroblasts and n = 10 for lung fibroblasts) were seeded in 6-well plates and stimulated with TGF-β isoforms 1, 2, and 3 for 72 h. Cell-free supernatant was collected and analyzed using ELISA to measure the concentration of ( A – C ) IL-11, ( D – F ) IL-6, and ( G – I ) IL-8. Data represent the mean and standard error of the mean (SEM). t -tests and ANOVA with Tukey’s post hoc test between groups were used to test for differences between groups. * ( p < 0.05), ** ( p < 0.01), *** ( p < 0.001), **** ( p < 0.0001).

Article Snippet: As shown in , the study design used neonatal dermal fibroblasts (BJ, Cat: CRL-2522) and fetal lung fibroblasts (HFL1, Cat: CCL-153) obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA), which are commonly used human, non-transformed dermal and lung fibroblast cell lines.

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay

Journal: Cells

Article Title: Systematic Comparison of the TGF-β Isoforms in Normal Dermal and Lung Fibroblasts Identifies TGF-β2 and TGF-β3 as Priority Targets in Tissue Fibrosis

doi: 10.3390/cells15080671

Figure Lengend Snippet: TGF-β2 and TGF-β3 are strong inducers of extracellular matrix production by dermal and lung fibroblasts. Dermal and lung fibroblasts ( n = 10 biological replicates per cell line) were seeded in 6-well plates and stimulated with TGF-β isoforms 1, 2, and 3 for 72 h. Protein lysates were collected and analyzed using Western blotting to measure expression of ( A – C ) collagen-I and ( D – F ) fibronectin. Data represent the mean with SEM. t -tests and ANOVA with Tukey’s post hoc test between groups were used to test for differences between groups. * ( p < 0.05), ** ( p < 0.01), *** ( p < 0.001), **** ( p < 0.0001).

Article Snippet: As shown in , the study design used neonatal dermal fibroblasts (BJ, Cat: CRL-2522) and fetal lung fibroblasts (HFL1, Cat: CCL-153) obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA), which are commonly used human, non-transformed dermal and lung fibroblast cell lines.

Techniques: Western Blot, Expressing

Journal: Cells

Article Title: Systematic Comparison of the TGF-β Isoforms in Normal Dermal and Lung Fibroblasts Identifies TGF-β2 and TGF-β3 as Priority Targets in Tissue Fibrosis

doi: 10.3390/cells15080671

Figure Lengend Snippet: TGF-β2 and TGF-β3 are strong inducers of fibroblast-to-myofibroblast differentiation in dermal and lung fibroblasts. Dermal and lung fibroblasts ( n = 10 biological replicates per cell line) were seeded in 6-well plates and stimulated with TGF-β isoforms 1, 2, and 3 for 72 h. Protein lysates were collected and analyzed using Western blotting to measure expression of α-smooth muscle actin of dermal and lung fibroblasts at baseline ( A ), dermal fibroblasts stimulated with the TGF-β isoforms ( B ), and lung fibroblasts stimulated with the TGF-β isoforms ( C ). Data represent the mean with SEM. t -tests and ANOVA with Tukey’s post hoc test between groups were used to test for differences between groups. * ( p < 0.05), ** ( p < 0.01).

Article Snippet: As shown in , the study design used neonatal dermal fibroblasts (BJ, Cat: CRL-2522) and fetal lung fibroblasts (HFL1, Cat: CCL-153) obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA), which are commonly used human, non-transformed dermal and lung fibroblast cell lines.

Techniques: Western Blot, Expressing

Journal: Cells

Article Title: Systematic Comparison of the TGF-β Isoforms in Normal Dermal and Lung Fibroblasts Identifies TGF-β2 and TGF-β3 as Priority Targets in Tissue Fibrosis

doi: 10.3390/cells15080671

Figure Lengend Snippet: TGF-β2 and TGF-β3 are strong inducers of fibroblast contraction by dermal fibroblasts in a fibrotic environment. Dermal and lung fibroblasts ( n = 5–8 biological replicates per cell line) were seeded on top of collagen-I gel matrices in 12-well plates and stimulated with TGF-β isoforms 1, 2, and 3 for 72 h. Collagen-I gel contraction was measured for gels at ( A – C ) 0.4 mg/mL and ( D – F ) 0.8 mg/mL. Data represent the mean with SEM. t -tests and ANOVA with Tukey’s post hoc test between groups were used to test for differences between groups. * ( p < 0.05), ** ( p < 0.01).

Article Snippet: As shown in , the study design used neonatal dermal fibroblasts (BJ, Cat: CRL-2522) and fetal lung fibroblasts (HFL1, Cat: CCL-153) obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA), which are commonly used human, non-transformed dermal and lung fibroblast cell lines.

Techniques:

Journal: Cells

Article Title: Systematic Comparison of the TGF-β Isoforms in Normal Dermal and Lung Fibroblasts Identifies TGF-β2 and TGF-β3 as Priority Targets in Tissue Fibrosis

doi: 10.3390/cells15080671

Figure Lengend Snippet: The TGF-β isoforms do not influence proliferation of dermal and lung fibroblasts. Dermal and lung fibroblasts ( n = 6–8 biological replicates per cell line) were seeded in 6-well plates and stimulated with TGF-β isoforms 1, 2, and 3 and PDGF-BB for 72 h. Fibroblasts were detached and counted with an automated cell counter, normalized with the control treatment group, and reported as ( A ) number of cells or ( B , C ) fold change in proliferation. Data represent the mean with SEM. t -tests and ANOVA with Tukey’s post hoc test between groups were used to test for differences between groups. * ( p < 0.05), *** ( p < 0.001).

Article Snippet: As shown in , the study design used neonatal dermal fibroblasts (BJ, Cat: CRL-2522) and fetal lung fibroblasts (HFL1, Cat: CCL-153) obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA), which are commonly used human, non-transformed dermal and lung fibroblast cell lines.

Techniques: Control

Journal: Cells

Article Title: Systematic Comparison of the TGF-β Isoforms in Normal Dermal and Lung Fibroblasts Identifies TGF-β2 and TGF-β3 as Priority Targets in Tissue Fibrosis

doi: 10.3390/cells15080671

Figure Lengend Snippet: The TGF-β isoforms are strong inducers of canonical TGF-β signalling in dermal and lung fibroblasts. Dermal and lung fibroblasts ( n = 6 biological replicates per cell line) were seeded in 6-well plates and stimulated with TGF-β isoforms 1, 2, and 3 for 30 min or 72 h. Protein lysates were collected and analyzed using Western blotting to measure expression of TGF-βRII ( A – C ), SMAD7 ( D – F ), and SMAD2/3 ( G – I ). Data represent the mean with SEM. t -tests and ANOVA with Tukey’s post hoc test between groups were used to test for differences between groups. * ( p < 0.05), ** ( p < 0.01), *** ( p < 0.001).

Article Snippet: As shown in , the study design used neonatal dermal fibroblasts (BJ, Cat: CRL-2522) and fetal lung fibroblasts (HFL1, Cat: CCL-153) obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA), which are commonly used human, non-transformed dermal and lung fibroblast cell lines.

Techniques: Western Blot, Expressing

Journal: Cells

Article Title: Systematic Comparison of the TGF-β Isoforms in Normal Dermal and Lung Fibroblasts Identifies TGF-β2 and TGF-β3 as Priority Targets in Tissue Fibrosis

doi: 10.3390/cells15080671

Figure Lengend Snippet: The TGF-β isoforms may have differential effects on non-canonical TGF-β signalling in dermal and lung fibroblasts. Dermal and lung fibroblasts ( n = 5 biological replicates per cell line) were seeded in 6-well plates and stimulated with TGF-β isoforms 1, 2, and 3 for 6 h. Protein lysates were collected and analyzed using Western blotting to measure expression of p38 ( A – C ) and ERK1/2 ( D – F ). Data represent the mean with SEM. t -tests and ANOVA with Tukey’s post hoc test between groups were used to test for differences between groups. * ( p < 0.05).

Article Snippet: As shown in , the study design used neonatal dermal fibroblasts (BJ, Cat: CRL-2522) and fetal lung fibroblasts (HFL1, Cat: CCL-153) obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA), which are commonly used human, non-transformed dermal and lung fibroblast cell lines.

Techniques: Western Blot, Expressing